Single Cell Metabolism
SCENITH™ is a method to functionally profile metabolism in multiple cells in parallel by flow cytometry.
We developed protocols for different type of samples, a full set of multiple metabolic inhibitors and an adapted monoclonal antibody.
We will let you know about the upcoming releases and other exciting developments. Your contributions have been essential to our journey, and we can't wait to have you as one of the initial users to experience
SCENITH™ firsthand.
Noella Lopes, PhD
We used the SCENITH kit and this allowed us to demonstrate the metabolic dichotomy between gamma delta T cell subsets in the thymus, periphery but also in the tumor microenvironment *1. The kit is easy to use and very useful to study the metabolic profile of cells in rare cell populations. We obtained very rapidly and in a simple way a large amount of information about metabolism like glycolytic capacity, glucose/mitochondria dependency, fatty acid oxidation capacity. SCENITH is the best technology to analyze and deepen our knowledge in the metabolism field, specially for rare but important immune cells types
Lopes, N., McIntyre, C., Martin, S. et al. Distinct metabolic programs established in the thymus control effector functions of γδ T cell subsets in tumor microenvironments.
Nat Immunol (2021). https://doi.org/10.1038/s41590-020-00848-3
SCENITH™ Publications
2023
Title: Light-inducible T cell engagers trigger, tune and shape the activation of primary T cells.
Authors: Jaeger M, Anastasio A, Brustlein S, Vincentelli R, Durbesson F, Char R, Boussand M, Lechelon M, Argüello RJ, Marguet D, He HT, Lasserre R.
Journal: PNAS (In Press) 2023. Link
Impact Factor (IF): 11
Title: A novel inborn error of immunity with biallelic mutations in NFATC1 impairing the CD8+ T-cell function via perturbed glycolysis.
Authors: Kostel Ba S, Giuliani S, Block J, Repiscak P, Shahin T, Kasap N, Ransmayr B, Hafemeister C, Miao Y, van de Wetering C, Frohne A, Jimenez Heredia R, Schuster M, Zoghi S, Hertlein V, Thian M, Babayeva R, Bilgic Eltan S, Karakoc Aydiner E, Shaw LA, Chowdhury I, Varjosalo M, Argüello RJ, Farlik M, Ozen A, Serfling E, Dupre L, Halbritter F, Bock C, Hannich JT, Castanon I, Kraakman MJ, Baris S, Boztug K.
Journal: Blood 2023. Link
Impact Factor (IF): 25.7
Title: A Deeply Quiescent Subset of CML LSC Depend on FAO Yet Avoid Deleterious ROS by Suppressing Mitochondrial Complex I.
Authors: Chimge NO, Chen MH, Nguyen Cu, Wu X, Garcia Gonzalez P, Ogana H, Hurwitz S, Teo JL, Chen X, Jin V, Du J, Kim YM, Ono M, Argüello RJ, Michael Kahn.
Journal: Current Mol. Pharmacology 2023.
Impact Factor (IF): 4
2022
Title: Distinct Metabolic States Guide Maturation of Inflammatory and Tolerogenic Dendritic Cells.
Authors: Adamik J, Munson P, Hartmann FJ, Combes AJ, Pierre P, Krummel MF, Bendall SC, Argüello RJ*, Butterfield LH*.(*co-last)
Journal: Nature Communications 2022. Link
Impact Factor (IF): 14.9
Title: OXPHOS Promotes Apoptotic Resistance and Persistence in T-H17 cells.
Authors: Hong HS, Mbah NE, Shan M, Loesel K, Lin L, Sajjakulnukit P, Correa LO, Andren A, Lin J, Hayashi A, Magnuson B, Li Z, Xie Y, Zhang L, Lei YL, Opipari AW, Argüello RJ, Kryczek I, Kamada N, Zou W, Franchi L, Costas A. Lyssiotis.
Journal: Science Immunology 2022. Link
Impact Factor (IF): 17.7
Title: Transcriptional reprogramming from innate immune functions to a pro-thrombotic signature upon SARS-CoV-2 sensing by monocytes in COVID-19.
Authors: Maher AK, Burnham KL, Jones E, Tan MMH, Saputil RC, Baillon L, Selck C, Giang N, Argüello RJ, Pillay C, Thorley, E, Short CE, Quinlan R, Barclay WS, Cooper N, Taylor GP, Davenport EE, Dominguez-Villar M.
Journal: Nature Communications 2022. Link
Impact Factor (IF): 14.9
Title: Dysfunctional subsets of CD39+ T cells, distinct from PD-1+, driven by leukemic extracellular vesicles in myeloid leukemias.
Authors: Swatler J, Lo Tartaro D, Borella R, Brewinska-Olchowik M, Paolini AM, Neroni A, Turos-Korgul L, Wiech M, Kozlowska E, Cysewski D, Grabowska-Pyrewicz W, Wodjda U, Basak G, Argüello RJ, Cossarizza A, De Biasi S, Piwocka K.
Journal: Haematologica 2022. Link
Impact Factor (IF): 11
Title: Rewiring the altered tryptophan metabolism as a novel therapeutic strategy in inflammatory bowel diseases.
Authors: Michaudel C, Danne C, Agus A, Magniez A, Aucouturier A, Spatz M, Lefevre A, Kirchgesner J, Rolhion N, Wang Y, Lavelle A, Galbert C, Da-Costa G, Poirier M, Lapiere A, Planchais J, Nádvorník P, Illes P, Oeuvray C, Michel ML, Benech N, Bourrier A, Nion-Larmurier A, Landman C, Richard ML, Emond P, Seksik P, Beaugerie L, Argüello RJ, Moulin D, Mani S, Dvorák Z, Bermúdez-Humarán LG, Langella P, Sokol H.
Journal: Gut 2022. Link
Impact Factor (IF): 31.8
Title: Unravelling the sex-specific diversity and functions of adrenal gland macrophages.
Authors: Dolfi B, Gallerand A, MM Firulyova, Y Xu, J Merlin, A Dumont, A Castiglione, N Vaillant, S Quemener, H Gerke, MI. Stunault, PR Schrank, SH Kim, A Zhu, J Ding, J Gilleron, V Magnone, P Barbry, D Dombrowicz, C Duranton, A Wakkach, C Blin-Wakkach, B Becher, S Pagnotta, Argüello RJ, P Rantakari, S Chakarov, F Ginhoux, K Zaitsev, KW Kim, LY Charvet, RR Ginamard, JW Williams, S Ivanov.
Journal: Cell Reports 2022. Link
Impact Factor (IF): 9.4
Title: ILC precursors differentiate into metabolically distinct ILC1-like cells during Mycobacterium tuberculosis infection.
Authors: Corral D, Charton A, Krauss MZ, Blanquart E, Levillain F, Lefrançais E, Sneperger T, Girard J-P, Eberl G, Poquet Y, Guéry JC, Argüello RJ, Belkaid Y, Hepworth MR, Neyrolles O, Hudrisier D.
Journal: Cell Reports 2022. Link
Impact Factor (IF): 9.4
Title: A toolbox to profile immunometabolism tested in macrophages.
Authors: Verberk SGS, de Goede KE, Gorki FS, van Dierendonck X, Argüello RJ, Van den Bossche J.
Journal: Cell Reports Methods 2022. Link
Impact Factor (IF): 10.6
2021
Title: Mitochondrial inhibitors circumvent adaptive resistance to venetoclax and cytarabine combination therapy in acute myeloid leukemia.
Authors: Bosc C, Saland E, Bousard A, Gadaud N, Sabatier M, Cognet G, Farge T, Boet E, Gotanègre M, Aroua N, Mouchel PL, Polley N, Larrue C, Kaphan E, Argüello RJ, Mazzotti C, Avet-Loiseau, Vergez F, Tamburini J, Fournié JJ, Tion IS, Wei AH, Kaoma T, Marine JC, Récher C, Stuani L, Joffre C, Sarry JE.
Journal: Nature Cancer 2021. Link
Impact Factor (IF): 24
Title: Distinct metabolic programmes control the effector fate of γδ T cell subsets and their activities in the tumour microenvironment.
Authors: Lopes N, McIntyre C, Martin S, Raverdeau M, Sumaria N, Kohlgruber AC, Agudelo L, Dyck L, Kane H, Douglas A, Cunningham S, Prendeville S, Loftus R, Carmody C, Pierre P, Kellis M, Brenner M, Argüello RJ, Silva-Santos B*, Pennington DJ*, Lynch L*.
Journal: Nature Immunology 2021. Link
Impact Factor (IF): 27
Title: SCENITH: A flow cytometry-based method for functional profiling energy metabolism with single cell resolution.
Authors: Argüello RJ@*, Combes AJ, Char R, Bousiquot E, Gigan JP, Camosseto V, Samad B, Tsui J, Yan P, Boissonneau S, Figarella-Branger D.F, Tabouret E, Gatti E, Krummel MF, Pierre P.
Journal: Cell Metabolism 2020. Link
Impact Factor (IF): 31
"Single Cell
Metabolism
by SCENITH"
© Olivier Cabaud
